Coding

Part:BBa_K4693001:Design

Designed by: Umashankar Chellam   Group: iGEM23_IISER-TVM   (2023-10-09)


Gsalpha


Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    COMPATIBLE WITH RFC[12]
  • 21
    INCOMPATIBLE WITH RFC[21]
    Illegal BamHI site found at 1202
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    COMPATIBLE WITH RFC[25]
  • 1000
    COMPATIBLE WITH RFC[1000]


Design Notes

We needed to change the DNA code of the reverse-translated protein sequences from UniProt for E. coli, and add spacer sequences at the beginning and end so that we could add our restriction sites Nde1 and Bamh1.


Source

The protein sequence of the part was sourced from uniprot and using reverse complement tool we obtained the Fasta sequence of Gsalpha: https://www.uniprot.org/uniprotkb/P63092/entry

References